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验证数据展示

  • WB analysis using 85703-1-RR

    WB analysis using 85703-1-RR

    Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85703-1-RR (Phospho-ATG4B (Ser383) antibody) blocked with BSA only, Phospho-ATG4B (Ser383) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • FC experiment of K-562 using 85703-1-RR

    FC experiment of K-562 using 85703-1-RR

    1X10^6 K-562 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ATG4B (Ser383) Recombinant monoclonal antibody (85703-1-RR, Clone:242927A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-ATG4B (Ser383) Recombinant monoclonal antibody

Phospho-ATG4B (Ser383) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85703-1-RR

产品说明书

CloneNo. 242927A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

P ATG4B (Ser383), P-ATG4B (Ser383), Phos ATG4B (Ser383), Phos-ATG4B (Ser383), ATG4B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive FC (Intra) detected inλ phosphatase treated K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85703-1-RR targets Phospho-ATG4B (Ser383) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATG4 autophagy related 4 homolog B (S. cerevisiae)
别名 P ATG4B (Ser383), P-ATG4B (Ser383), Phos ATG4B (Ser383), Phos-ATG4B (Ser383), ATG4B
计算分子量 44 kDa
观测分子量44-48 kDa
GenBank蛋白编号BC000719
基因名称 ATG4B
Gene ID (NCBI) 23192
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4P1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)

实验方案

Product Specific Protocols
FC protocol for Phospho-ATG4B (Ser383) antibody 85703-1-RRDownload protocol
WB protocol for Phospho-ATG4B (Ser383) antibody 85703-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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