• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 85703-1-RR (same clone as 85703-1-PBS)

    WB analysis using 85703-1-RR (same clone as 85703-1-PBS)

    Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85703-1-RR (Phospho-ATG4B (Ser383) antibody) blocked with BSA only, Phospho-ATG4B (Ser383) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85703-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 85703-1-RR (same clone as 85703-1-PBS)

    FC experiment of K-562 using 85703-1-RR (same clone as 85703-1-PBS)

    1X10^6 K-562 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ATG4B (Ser383) Recombinant monoclonal antibody (85703-1-RR, Clone:242927A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 85703-1-PBS in a different storage buffer formulation.

Phospho-ATG4B (Ser383) Recombinant monoclonal antibody, PBS Only

Phospho-ATG4B (Ser383) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 85703-1-PBS

产品说明书

CloneNo. 242927A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

P ATG4B (Ser383), P-ATG4B (Ser383), Phos ATG4B (Ser383), Phos-ATG4B (Ser383), ATG4B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

85703-1-PBS targets Phospho-ATG4B (Ser383) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATG4 autophagy related 4 homolog B (S. cerevisiae)
别名 P ATG4B (Ser383), P-ATG4B (Ser383), Phos ATG4B (Ser383), Phos-ATG4B (Ser383), ATG4B
计算分子量 44 kDa
观测分子量44-48 kDa
GenBank蛋白编号BC000719
基因名称 ATG4B
Gene ID (NCBI) 23192
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4P1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)

||
New chat Able
正在加载，请稍候...