验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | K-562 cells |
For other applications, we recommend the unconjugated version of this antibody, 85703-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-85703 targets Phospho-ATG4B (Ser383) in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ATG4 autophagy related 4 homolog B (S. cerevisiae) |
| 别名 | ATG4B, APG4B, AUTL1, AUT-like 1 cysteine endopeptidase, Autophagin-1 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44-48 kDa |
| GenBank蛋白编号 | BC000719 |
| 基因名称 | ATG4B |
| Gene ID (NCBI) | 23192 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Y4P1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 Phospho-ATG4B (Ser383) antibody CL647-85703 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
