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验证数据展示

  • FC experiment of HeLa using 85177-3-RR

    FC experiment of HeLa using 85177-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HUWE1 Recombinant antibody (85177-3-RR, Clone:242702F10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 85177-3-RR

    WB analysis using 85177-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85177-3-RR (HUWE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 85177-3-RR

    IHC staining of mouse testis using 85177-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HUWE1 antibody included
  • IHC staining of human rectal cancer using 85177-3-RR

    IHC staining of human rectal cancer using 85177-3-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HUWE1 antibody included
  • IHC staining of human rectal cancer using 85177-3-RR

    IHC staining of human rectal cancer using 85177-3-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HUWE1 antibody included
  • Affinity and Kinetic Characterization of 85177-3-RR

    Affinity and Kinetic Characterization of 85177-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85177-3-RR against Human HUWE1 were performed. The affinity constant is 26.1 nM.

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HUWE1 Recombinant monoclonal antibody

HUWE1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 85177-3-RR

产品说明书

CloneNo. 242702F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

242702F10, ARF binding protein 1, ARF BP1, ARF-binding protein 1, ARF-BP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, NIH/3T3 cells
Positive IHC detected inhuman rectal cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HUWE1 Ready-To-Use IHC Kit. HUWE1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85177-3-RR targets HUWE1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13763

Product name: Recombinant human HUWE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3983-4374 aa of BC002602

Sequence: AVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVLRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HECT, UBA and WWE domain containing 1
别名 242702F10, ARF binding protein 1, ARF BP1, ARF-binding protein 1, ARF-BP1
计算分子量 437 aa, 482 kDa
观测分子量482 kDa
GenBank蛋白编号BC002602
基因名称 HUWE1
Gene ID (NCBI) 10075
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z6Z7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).

实验方案

Product Specific Protocols
WB protocol for HUWE1 antibody 85177-3-RRDownload protocol
IHC protocol for HUWE1 antibody 85177-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols