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验证数据展示

  • FC experiment of HeLa using 85177-3-RR (same clone as 85177-3-PBS)

    FC experiment of HeLa using 85177-3-RR (same clone as 85177-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HUWE1 Recombinant antibody (85177-3-RR, Clone:242702F10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

  • WB analysis using 85177-3-RR (same clone as 85177-3-PBS)

    WB analysis using 85177-3-RR (same clone as 85177-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85177-3-RR (HUWE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 85177-3-RR (same clone as 85177-3-PBS)

    IHC staining of mouse testis using 85177-3-RR (same clone as 85177-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HUWE1 antibody included
  • IHC staining of human rectal cancer using 85177-3-RR (same clone as 85177-3-PBS)

    IHC staining of human rectal cancer using 85177-3-RR (same clone as 85177-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HUWE1 antibody included
  • IHC staining of human rectal cancer using 85177-3-RR (same clone as 85177-3-PBS)

    IHC staining of human rectal cancer using 85177-3-RR (same clone as 85177-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85177-3-RR (HUWE1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HUWE1 antibody included
  • IF Staining of NIH/3T3 using 85177-3-RR (same clone as 85177-3-PBS)

    IF Staining of NIH/3T3 using 85177-3-RR (same clone as 85177-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using HUWE1 antibody (85177-3-RR, Clone: 242702F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85177-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85177-3-RR

    Affinity and Kinetic Characterization of 85177-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85177-3-RR against Human HUWE1 were performed. The affinity constant is 26.1 nM.

HUWE1 Recombinant monoclonal antibody, PBS Only

HUWE1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85177-3-PBS

产品说明书

CloneNo. 242702F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

242702F10, ARF binding protein 1, ARF BP1, ARF-binding protein 1, ARF-BP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HUWE1 Ready-To-Use IHC Kit. HUWE1 primary antibody included.

产品信息

85177-3-PBS targets HUWE1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13763

Product name: Recombinant human HUWE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3983-4374 aa of BC002602

Sequence: AVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVLRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HECT, UBA and WWE domain containing 1
别名 242702F10, ARF binding protein 1, ARF BP1, ARF-binding protein 1, ARF-BP1
计算分子量 437 aa, 482 kDa
观测分子量482 kDa
GenBank蛋白编号BC002602
基因名称 HUWE1
Gene ID (NCBI) 10075
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z6Z7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).

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