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验证数据展示

  • WB analysis using 84564-6-RR

    WB analysis using 84564-6-RR

    T-47D cell lysates were subjected to SDS PAGE followed by western blot with 84564-6-RR (Phospho-Estrogen Receptor α (Ser118) antibody) blocked with BSA only, Phospho-Estrogen Receptor α (Ser118) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • FC experiment of MCF-7 using 84564-6-RR

    FC experiment of MCF-7 using 84564-6-RR

    1X10^6 MCF-7 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody (84564-6-RR, Clone:250278F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody

Phospho-Estrogen Receptor α (Ser118) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84564-6-RR

产品说明书

CloneNo. 250278F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Estrogen Receptor α (Ser118), p Estrogen Receptor α, p-ERS1, p-ERS1 (Ser118), p-Estrogen Receptor α

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inT-47D cells
Positive FC (Intra) detected inλ phosphatase treated MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84564-6-RR targets Phospho-Estrogen Receptor α (Ser118) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 estrogen receptor 1
别名 Estrogen Receptor α (Ser118), p Estrogen Receptor α, p-ERS1, p-ERS1 (Ser118), p-Estrogen Receptor α
计算分子量 595 aa, 66 kDa
观测分子量66 kDa
GenBank蛋白编号BC128573
基因名称 ER
Gene ID (NCBI) 2099
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP03372
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)

实验方案

Product Specific Protocols
FC protocol for Phospho-Estrogen Receptor α (Ser118) antibody 84564-6-RRDownload protocol
WB protocol for Phospho-Estrogen Receptor α (Ser118) antibody 84564-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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