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验证数据展示

  • WB analysis using 84564-6-RR (same clone as 84564-6-PBS)

    WB analysis using 84564-6-RR (same clone as 84564-6-PBS)

    T-47D cell lysates were subjected to SDS PAGE followed by western blot with 84564-6-RR (Phospho-Estrogen Receptor α (Ser118) antibody) blocked with BSA only, Phospho-Estrogen Receptor α (Ser118) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84564-6-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 84564-6-RR (same clone as 84564-6-PBS)

    FC experiment of MCF-7 using 84564-6-RR (same clone as 84564-6-PBS)

    1X10^6 MCF-7 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody (84564-6-RR, Clone:250278F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 84564-6-PBS in a different storage buffer formulation.

Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody, PBS Only

Phospho-Estrogen Receptor α (Ser118) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84564-6-PBS

产品说明书

CloneNo. 250278F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

Estrogen Receptor α (Ser118), p Estrogen Receptor α, p-ERS1, p-ERS1 (Ser118), p-Estrogen Receptor α

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84564-6-PBS targets Phospho-Estrogen Receptor α (Ser118) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 estrogen receptor 1
别名 Estrogen Receptor α (Ser118), p Estrogen Receptor α, p-ERS1, p-ERS1 (Ser118), p-Estrogen Receptor α
计算分子量 595 aa, 66 kDa
观测分子量66 kDa
GenBank蛋白编号BC128573
基因名称 ER
Gene ID (NCBI) 2099
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP03372
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)

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