验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 84564-6-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84564-6 targets Phospho-Estrogen Receptor α (Ser118) in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | estrogen receptor 1 |
| 别名 | Estrogen Receptor α (Ser118), p Estrogen Receptor α, p-ERS1, p-ERS1 (Ser118), p-Estrogen Receptor α |
| 计算分子量 | 595 aa, 66 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC128573 |
| 基因名称 | ER |
| Gene ID (NCBI) | 2099 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P03372 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Phospho-Estrogen Receptor α (Ser118) antibody CL488-84564-6 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
