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验证数据展示

  • ChIP experiment of HT-1080 using 82016-1-RR

    ChIP experiment of HT-1080 using 82016-1-RR

    Chromatin was prepared from HT-1080 cells either non-treated or treated with hIFN-γ (50 ng/ml, 30min). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of STAT1 (82016-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis using 82016-1-RR

    WB analysis using 82016-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 82016-1-RR

    IHC staining of human ovary tumor using 82016-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT1 antibody included
  • IHC staining of human ovary tumor using 82016-1-RR

    IHC staining of human ovary tumor using 82016-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT1 antibody included
  • WB analysis of HEK-293 using 82016-1-RR

    WB analysis of HEK-293 using 82016-1-RR

    WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells.

  • IP experiment of PC-3 using 82016-1-RR

    IP experiment of PC-3 using 82016-1-RR

    IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug.

  • IF Staining of HeLa using 82016-1-RR

    IF Staining of HeLa using 82016-1-RR

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82016-1-RR

    Affinity and Kinetic Characterization of 82016-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

  • Featured Product
  • KD/KO验证

STAT1 Recombinant monoclonal antibody

STAT1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR
Cat No. 82016-1-RR

产品说明书

CloneNo. 2F16
引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR

别名

2F16, ISGF 3, Signal transducer and activator of transcription 1-alpha/beta, STAT91, Transcription factor ISGF-3 components p91/p84

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman ovary tumor tissue, mouse spleen tissue, mouse colon tissue, rat colon tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inIFN alpha treated HeLa cells
Positive ChIP-qPCR detected inIFN-γ -HT-1080 (50 ng/ml, 30min) HT-1080 cells
Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

82016-1-RR targets STAT1 in WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag0199

Product name: Recombinant human STAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-230 aa of BC002704

Sequence: SQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal transducer and activator of transcription 1, 91kDa
别名 2F16, ISGF 3, Signal transducer and activator of transcription 1-alpha/beta, STAT91, Transcription factor ISGF-3 components p91/p84
计算分子量 83 kDa
观测分子量 84 kDa
GenBank蛋白编号BC002704
基因名称 STAT1
Gene ID (NCBI) 6772
RRIDAB_2935597
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42224
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.

实验方案

Product Specific Protocols
WB protocol for STAT1 antibody 82016-1-RRDownload protocol
IHC protocol for STAT1 antibody 82016-1-RRDownload protocol
IF protocol for STAT1 antibody 82016-1-RRDownload protocol
IP protocol for STAT1 antibody 82016-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Noncoding RNA Res

LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population

Authors - Hongjiao Wu
View Article
humanWB

Cell Biochem Biophys

FOXA1 Transcriptional Repression of PLSCR1 Inhibits Tongue Squamous Cell Carcinoma Progression

Authors - Jinxin Wang
View Article