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验证数据展示

  • ChIP experiment of HT-1080 using 82016-1-RR (same clone as 82016-1-PBS)

    ChIP experiment of HT-1080 using 82016-1-RR (same clone as 82016-1-PBS)

    Chromatin was prepared from HT-1080 cells either non-treated or treated with hIFN-γ (50 ng/ml, 30min). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of STAT1 (82016-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • WB analysis using 82016-1-RR (same clone as 82016-1-PBS)

    WB analysis using 82016-1-RR (same clone as 82016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT1 antibody included
  • IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT1 antibody included
  • WB analysis of HEK-293 using 82016-1-RR (same clone as 82016-1-PBS)

    WB analysis of HEK-293 using 82016-1-RR (same clone as 82016-1-PBS)

    WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 82016-1-RR (same clone as 82016-1-PBS)

    IP experiment of PC-3 using 82016-1-RR (same clone as 82016-1-PBS)

    IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82016-1-RR (same clone as 82016-1-PBS)

    IF Staining of HeLa using 82016-1-RR (same clone as 82016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82016-1-RR

    Affinity and Kinetic Characterization of 82016-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

  • Featured Product
  • KD/KO验证
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STAT1 Recombinant monoclonal antibody, PBS Only

STAT1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA, ChIP-qPCR
Cat No. 82016-1-PBS

产品说明书

CloneNo. 2F16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA, ChIP-qPCR

别名

2F16, ISGF 3, Signal transducer and activator of transcription 1-alpha/beta, STAT91, Transcription factor ISGF-3 components p91/p84

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

产品信息

82016-1-PBS targets STAT1 in WB, IHC, IF/ICC, IP, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0199

Product name: Recombinant human STAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-230 aa of BC002704

Sequence: SQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal transducer and activator of transcription 1, 91kDa
别名 2F16, ISGF 3, Signal transducer and activator of transcription 1-alpha/beta, STAT91, Transcription factor ISGF-3 components p91/p84
计算分子量 83 kDa
观测分子量 84 kDa
GenBank蛋白编号BC002704
基因名称 STAT1
Gene ID (NCBI) 6772
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42224
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.