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验证数据展示

  • IF Staining of rat pancreas using 60931-1-Ig

    IF Staining of rat pancreas using 60931-1-Ig

    FFPE-rat pancreas tissue was stained with GLP1R monoclonal antibody (Cat.NO. 60931-1-Ig) at 1:500 followed by detection with Multi-rAb® Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) (Cat.NO. RGAU011). CoraLite®Plus 647-Tyramide Reagent (Cat.NO. PR30025) was used for signal development (purple). The slide was then conducted HIER in pH 9.0 Tris-EDTA buffer followed by staining with INS monoclonal antibody (66198-1-Ig, 1:5000). CoraLite®Plus 488-Tyramide Reagent (Cat.NO. PR30022) were used for signal development (green). Cell nucleus (blue) were stained with DAPI. The signal from each channel was captured and merged.

GLP1R Monoclonal antibody

GLP1R Monoclonal Antibody for IF/ICC, ELISA
Cat No. 60931-1-Ig

产品说明书

CloneNo. 1A4G5

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

IF/ICC, ELISA

别名

GLP 1 R, GLP 1 receptor, GLP 1R, GLP-1 receptor, GLP-1R

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inrat pancreas tissue

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60931-1-Ig targets GLP1R in IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag23529

Product name: Recombinant human GLP1R protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 26-145 aa of BC112126

Sequence: QGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLFLY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glucagon-like peptide 1 receptor
别名 GLP 1 R, GLP 1 receptor, GLP 1R, GLP-1 receptor, GLP-1R
计算分子量 463 aa, 53 kDa
GenBank蛋白编号BC112126
基因名称 GLP1R
Gene ID (NCBI) 2740
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP43220
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLP1R (glucagon-like peptide-1 receptor) is a G protein-coupled receptor belonging to the secretin receptor superfamily, also known as class B (PMID:34911028). GLP1R is widely distributed in pancreatic islets, muscles, the gastrointestinal tract, the lung, liver, pancreas, and other tissues or organs (PMID:35989517). Moreover, GLP1R agonist has been reported to induce autophagy of EC (Endometrial carcinoma) cells, and high GLP1R expression may be associated with good prognosis of EC patients, suggesting that GLP1R is likely to participate in the progression of EC (PMID:29907137). Moreover, regarding the molecular weight of GLP1R, research shows that the bands at ~50 kDa and ~100 kDa belong to the monomer and dimer states of GLP1R (PMID: 28609478).

实验方案

Product Specific Protocols
IF protocol for GLP1R antibody 60931-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols