Gamma Tubulin Monoclonal antibody

Gamma Tubulin Monoclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, Canine, mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3F9H8

Cat No : 66320-1-Ig

Print datasheet

Synonyms

Gamma 1 tubulin, Gamma Tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin, Tubulin gamma 1 chain, tubulin, gamma 1, tubulin-gamma



经过测试的应用

Positive WB detected inNCCIT cells, HepG2 cells, EC109 cells, K-562 cells, HSCT6 cells, NIH3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, HeLa cells, A549 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66320-1-Ig targets Gamma Tubulin in WB, IF, IHC, ELISA applications and shows reactivity with human, Canine, mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, Canine, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Gamma Tubulin fusion protein Ag23913 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name tubulin, gamma 1
Synonyms Gamma 1 tubulin, Gamma Tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin, Tubulin gamma 1 chain, tubulin, gamma 1, tubulin-gamma
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC000619
Gene Symbol TUBG1
Gene ID (NCBI) 7283
RRIDAB_2857350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

实验方案

Product Specific Protocols
WB protocol for Gamma Tubulin antibody 66320-1-IgDownload protocol
IHC protocol for Gamma Tubulin antibody 66320-1-IgDownload protocol
IF protocol for Gamma Tubulin antibody 66320-1-IgDownload protocol
FC protocol for Gamma Tubulin antibody 66320-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Structure of LRRK2 in Parkinson's disease and model for microtubule interaction.

Authors - C K Deniston
IF

EMBO Mol Med

Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signaling.

Authors - Michaela Bosakova
mouseWB

J Cell Biol

Time-resolved proteomics profiling of the ciliary Hedgehog response.

Authors - Elena A May
IF

Cell Rep

MYO10 regulates genome stability and cancer inflammation through mediating mitosis

Authors - Franklin Mayca Pozo
humanIF

Elife

Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production.

Authors - MengJie Hu
humanWB,IF

J Nanobiotechnology

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer.

Authors - Linjia Su