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p70(S6K) Polyclonal antibody

p70(S6K) Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 26587-1-AP

Print datasheet

Synonyms

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6k, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

验证数据展示

  • IHC staining of human lung cancer using 26587-1-AP

    IHC staining of human lung cancer using 26587-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human lung cancer using 26587-1-AP

    IHC staining of human lung cancer using 26587-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of MCF-7 using 26587-1-AP

    WB analysis of MCF-7 using 26587-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26587-1-AP (p70(S6K) Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 26587-1-AP

    IP experiment of A549 using 26587-1-AP

    IP result of anti-p70(S6K) (IP:26587-1-AP, 4ug; Detection:26587-1-AP 1:300) with A549 cells lysate 1600ug.

  • IF Staining of A549 using 26587-1-AP

    IF Staining of A549 using 26587-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26587-1-AP (p70(S6K) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 26587-1-AP is p70(S6K) Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitypig
Immunogen p70(S6K) fusion protein Ag24073 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Synonyms p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6k, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A
Calculated Molecular Weight 59 kDa
Observed Molecular Weight60-75 kDa
GenBank Accession NumberBC053365
Gene Symbol RPS6KB1
Gene ID (NCBI) 6198
RRIDAB_2880565
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59kDa produced by alternative initiation.

实验方案

Product Specific Protocols
WB protocol for p70(S6K) antibody 26587-1-APDownload protocol
IHC protocol for p70(S6K) antibody 26587-1-APDownload protocol
IF protocol for p70(S6K) antibody 26587-1-APDownload protocol
IP protocol for p70(S6K) antibody 26587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Am J Physiol Endocrinol Metab

Prematurity blunts the feeding-induced stimulation of translation initiation signaling and protein synthesis in muscle of neonatal piglets.

Authors - Jane K Naberhuis
View Article