验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | THP-1 cells |
Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-83078-5 targets p300 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | E1A binding protein p300 |
| 别名 | EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48 |
| 计算分子量 | 264 kDa |
| 观测分子量 | 300 kDa |
| GenBank蛋白编号 | NM_001429 |
| 基因名称 | EP300 |
| Gene ID (NCBI) | 2033 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q09472 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light.Stable for one year after shipment. |
背景介绍
p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE p300 antibody PE-83078-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
