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验证数据展示

  • IP experiment of HeLa using 83078-5-RR (same clone as 83078-5-PBS)

    IP experiment of HeLa using 83078-5-RR (same clone as 83078-5-PBS)

    IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83078-5-RR (same clone as 83078-5-PBS)

    IF Staining of A431 using 83078-5-RR (same clone as 83078-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 83078-5-RR (same clone as 83078-5-PBS)

    FC experiment of THP-1 using 83078-5-RR (same clone as 83078-5-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

p300 Recombinant antibody, PBS Only

p300 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 83078-5-PBS

产品说明书

CloneNo. 230369D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), IP, Indirect ELISA

别名

EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

产品信息

83078-5-PBS targets p300 in IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 E1A binding protein p300
别名 EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48
计算分子量 264 kDa
观测分子量300 kDa
GenBank蛋白编号NM_001429
基因名称 EP300
Gene ID (NCBI) 2033
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ09472
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).