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CoraLite® Plus 488-conjugated mDia1 Polyclonal antibody

mDia1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-20624

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

IF/ICC, FC (Intra)

DIAPH1, Diaphanous-related formin-1, Diaphanous related formin 1, DIAPH 1, DIAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DIAPH1 Ready-To-Use IHC Kit. DIAPH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20624-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20624 targets mDia1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, monkey
免疫原 mDia1 fusion protein Ag14523 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diaphanous homolog 1 (Drosophila)
别名 DIAPH1, Diaphanous-related formin-1, Diaphanous related formin 1, DIAPH 1, DIAP1
计算分子量 1272 aa, 141 kDa
观测分子量 140-150 kDa, 70 kDa
GenBank蛋白编号BC007411
基因名称 mDia1
Gene ID (NCBI) 1729
RRIDAB_3084036
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60610
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 mDia1 antibody CL488-20624Download protocol
FC protocol for CL Plus 488 mDia1 antibody CL488-20624Download protocol
Standard Protocols
Click here to view our Standard Protocols
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