• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated mDia1 Polyclonal antibody

mDia1 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-20624

Print datasheet

Synonyms

DFNA1, DIA1, DIAP1, DIAPH1, Diaphanous related formin 1, DRF1, FLJ25265, hDIA1, LFHL1, Protein diaphanous homolog 1

验证数据展示

  • IF Staining of HeLa using CL488-20624

    IF Staining of HeLa using CL488-20624

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 mDia1 antibody (CL488-20624) at dilution of 1:200.

  • FC experiment of HeLa using CL488-20624

    FC experiment of HeLa using CL488-20624

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human mDia1 (CL488-20624) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 20624-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20624 targets mDia1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat, monkey
Immunogen mDia1 fusion protein Ag14523 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diaphanous homolog 1 (Drosophila)
Synonyms DFNA1, DIA1, DIAP1, DIAPH1, Diaphanous related formin 1, DRF1, FLJ25265, hDIA1, LFHL1, Protein diaphanous homolog 1
Calculated Molecular Weight 1272 aa, 141 kDa
Observed Molecular Weight 140-150 kDa, 70 kDa
GenBank Accession NumberBC007411
Gene Symbol DIAPH1
Gene ID (NCBI) 1729
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60610
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 mDia1 antibody CL488-20624Download protocol
FC protocol for CL Plus 488 mDia1 antibody CL488-20624Download protocol
Standard Protocols
Click here to view our Standard Protocols