验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human heart tissue, human skeletal muscle tissue, COS-7 cells, NIH/3T3 cells, MDA-MB-231 cells, HUVEC cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20624-1-AP targets mDia1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | human, mouse |
| 免疫原 | mDia1 fusion protein Ag14523 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | diaphanous homolog 1 (Drosophila) |
| 别名 | DIAPH1, Diaphanous-related formin-1, Diaphanous related formin 1, DIAPH 1, DIAP1 |
| 计算分子量 | 1272 aa, 141 kDa |
| 观测分子量 | 140-150 kDa, 70 kDa |
| GenBank蛋白编号 | BC007411 |
| 基因名称 | mDia1 |
| Gene ID (NCBI) | 1729 |
| RRID | AB_10858618 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60610 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for mDia1 antibody 20624-1-AP | Download protocol |
| IHC protocol for mDia1 antibody 20624-1-AP | Download protocol |
| IF protocol for mDia1 antibody 20624-1-AP | Download protocol |
| IP protocol for mDia1 antibody 20624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Circ Res Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways. | ||
Dev Cell Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.
| ||
J Cell Biol Dia1 coordinates differentiation and cell sorting in a stratified epithelium. | ||
Mol Biol Cell Small molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy. | ||
Cells The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells. | ||
