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CoraLite®555-conjugated eIF4G2/DAP5 Monoclonal antibody

eIF4G2/DAP5 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3C11A3

Cat No : CL555-67428

Print datasheet

Synonyms

AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, eIF4G2, FLJ41344, NAT1, p97

验证数据展示

  • FC experiment of HeLa using CL555-67428

    FC experiment of HeLa using CL555-67428

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human eIF4G2/DAP5 (CL555-67428, Clone:3C11A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL555-67428

    IF Staining of HepG2 using CL555-67428

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 eIF4G2/DAP5 antibody (CL555-67428, Clone: 3C11A3 ) at dilution of 1:600.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EIF4G2 Ready-To-Use IHC Kit. EIF4G2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67428-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67428 targets eIF4G2/DAP5 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen eIF4G2/DAP5 fusion protein Ag12080 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4 gamma, 2
Synonyms AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, eIF4G2, FLJ41344, NAT1, p97
Calculated Molecular Weight 907 aa, 102 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC043149
Gene Symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2919702
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78344
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL555 eIF4G2/DAP5 antibody CL555-67428Download protocol
FC protocol for CL555 eIF4G2/DAP5 antibody CL555-67428Download protocol
Standard Protocols
Click here to view our Standard Protocols