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验证数据展示

  • ChIP experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

    ChIP experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of c-MYC (80845-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

    IF Staining of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 80845-1-RR (same clone as 80845-1-PBS)

    WB analysis of A549 using 80845-1-RR (same clone as 80845-1-PBS)

    WB result of MYC antibody (80845-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYC transfected A549 cells. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • WB analysis using 80845-1-RR (same clone as 80845-1-PBS)

    WB analysis using 80845-1-RR (same clone as 80845-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 80845-1-RR (c-MYC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 80845-1-RR (same clone as 80845-1-PBS)

    IHC staining of mouse embryo using 80845-1-RR (same clone as 80845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80845-1-RR (same clone as 80845-1-PBS)

    IHC staining of human tonsillitis using 80845-1-RR (same clone as 80845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80845-1-RR (same clone as 80845-1-PBS)

    IHC staining of human tonsillitis using 80845-1-RR (same clone as 80845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 80845-1-RR (same clone as 80845-1-PBS)

    IHC staining of human colon using 80845-1-RR (same clone as 80845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80845-1-RR (same clone as 80845-1-PBS)

    IHC staining of human colon cancer using 80845-1-RR (same clone as 80845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

    FC experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Recombinant antibody (80845-1-RR, Clone:2B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80845-1-RR

    Affinity and Kinetic Characterization of 80845-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80845-1-RR against Human c-MYC were performed. The affinity constant is 2.03 nM.

  • Featured Product
  • KD/KO验证

c-MYC Recombinant monoclonal antibody, PBS Only

c-MYC Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR
Cat No. 80845-1-PBS

产品说明书

CloneNo. 2B17

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

别名

MYC, 2B17, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80845-1-PBS targets c-MYC in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1263

Product name: Recombinant human MYC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 16-454 aa of BC000141

Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 MYC, 2B17, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39
计算分子量 49 kDa
观测分子量50-65 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
RRIDAB_2918915
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01106
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYC contains a basic helix-loop-helix (bHLH) domain. This protein is a multifunctional nuclear phosphoprotein, which plays a role in cell cycle process, apoptosis and cell transformation. As a transcription factor, it regulates the transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds to DNA in a nonspecific way, but also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutation, overexpression, rearrangement and translocation of this gene are related to many hematopoietic tumors, leukemia and lymphoma (including Burkitt lymphoma). The antibody is a rabbit polyclonal antibody against human MYC recombinant protein. The 50kDa band recognized by antibody is the natural form of MYC, while other bands between 60 and 70 kDa are the phosphorylated form of MYC (PMID: 12189186). The latest research reveals a new key mechanism of MYC protein, which is independent of its classical transcriptional regulation function. It was found that under the stress of transcription, MYC was polymerized through its specific RNA binding domain (RBRIII), and exocrine was targeted at the nuclear R loop and double-stranded RNA. This process can effectively eliminate the immunogenic RNA-DNA hybrid from R-loop, thus preventing it from activating TLR3-TBK1 natural immune signaling pathway. This mechanism is very important for MYC to maintain tumor growth and achieve immune escape in vivo. (PMID: 41576951)

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