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验证数据展示

  • ChIP experiment of HeLa using 80845-1-RR

    ChIP experiment of HeLa using 80845-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of c-MYC (80845-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • IF Staining of HeLa using 80845-1-RR

    IF Staining of HeLa using 80845-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of A549 using 80845-1-RR

    WB analysis of A549 using 80845-1-RR

    WB result of MYC antibody (80845-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYC transfected A549 cells.

  • WB analysis using 80845-1-RR

    WB analysis using 80845-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 80845-1-RR (c-MYC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 80845-1-RR

    IHC staining of mouse embryo using 80845-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 80845-1-RR

    IHC staining of human colon cancer using 80845-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 80845-1-RR

    FC experiment of HeLa using 80845-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Recombinant antibody (80845-1-RR, Clone:2B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 80845-1-RR

    Affinity and Kinetic Characterization of 80845-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80845-1-RR against Human c-MYC were performed. The affinity constant is 2.03 nM.

  • Featured Product
  • KD/KO验证

c-MYC Recombinant monoclonal antibody

c-MYC Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR
Cat No. 80845-1-RR

产品说明书

CloneNo. 2B17

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

别名

MYC, 2B17, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, HL-60 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells, U2OS cells
Positive FC (Intra) detected inHeLa cells
Positive ChIP-qPCR detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80845-1-RR targets c-MYC in WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1263

Product name: Recombinant human MYC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 16-454 aa of BC000141

Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 MYC, 2B17, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39
计算分子量 49 kDa
观测分子量50-65 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
RRIDAB_2918915
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01106
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

实验方案

Product Specific Protocols
IF protocol for c-MYC antibody 80845-1-RRDownload protocol
IHC protocol for c-MYC antibody 80845-1-RRDownload protocol
WB protocol for c-MYC antibody 80845-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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