CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for FC (Intra), IF, WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster, zebrafish, monkey, canine

Applications

FC (Intra), IF, WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D4H5

Cat No : CL488-66009

Print datasheet

Synonyms

ACTB, actin, actin, beta, Beta Actin



经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells
Positive IF detected inMDCK cells, U2OS cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66009-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66009 targets Beta Actin in WB, IF, FC (Intra) applications and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey, canine samples.

Tested Applications FC (Intra), IF, WB
Cited ApplicationsIF, WB
Tested Reactivity human, mouse, rat, hamster, zebrafish, monkey, canine
Cited Reactivityhuman, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name actin, beta
Synonyms ACTB, actin, actin, beta, Beta Actin
Calculated Molecular Weight 42 kDa
GenBank Accession NumberNM_001101
Gene Symbol ACTB
Gene ID (NCBI) 60
RRIDAB_2883225
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60709
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL488 (Ex/Em 488 nm/515 nm) conjugated.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Beta Actin antibody CL488-66009Download protocol
IF protocol for CL Plus 488 Beta Actin antibody CL488-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Br J Pharmacol

Fingolimod ameliorates schizophrenia-like cognitive impairments induced by phencyclidine in male rats

Authors - Xueli Yu
humanWB

Mediators Inflamm

Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2

Authors - Junqi Fu
humanWB

Oncol Lett

CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process

Authors - Yang Zhang
mouseIF

Nanoscale

Effects of hydrophilic fullerene nanoarchitectured structures on the behaviour of neural stem cells.

Authors - Chui-Wei Wong