CoraLite®594-conjugated band 3/ AE1 Monoclonal antibody
band 3/ AE1 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E5C1
验证数据展示
经过测试的应用
Positive IF detected in | human placenta tissue |
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66984-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66984 targets band 3/ AE1 in IF applications and shows reactivity with Human samples.
Tested Applications | IF-P |
Tested Reactivity | Human |
Immunogen | band 3/ AE1 fusion protein Ag28058 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group) |
Synonyms | AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, BND3, CD233, DI, EMPB3, EPB3, FR, RTA1A, SLC4A1, SW, WD, WD1, WR |
Calculated Molecular Weight | 911 aa, 102 kDa |
GenBank Accession Number | BC101570 |
Gene Symbol | SLC4A1 |
Gene ID (NCBI) | 6521 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P02730 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)
实验方案
Product Specific Protocols | |
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IF protocol for CL594 band 3/ AE1 antibody CL594-66984 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |