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CoraLite® Plus 488-conjugated band 3/ AE1 Monoclonal antibody

band 3/ AE1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E5C1

Cat No : CL488-66984

Print datasheet

Synonyms

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, BND3, CD233, DI, EMPB3, EPB3, FR, RTA1A, SLC4A1, SW, WD, WD1, WR

验证数据展示

  • IF Staining of human placenta using CL488-66984

    IF Staining of human placenta using CL488-66984

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 band 3/ AE1 antibody (CL488-66984, Clone: 1E5C1 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66984-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66984 targets band 3/ AE1 in IF applications and shows reactivity with Human samples.

Tested Applications IF-P
Tested Reactivity Human
Immunogen band 3/ AE1 fusion protein Ag28058 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Synonyms AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, BND3, CD233, DI, EMPB3, EPB3, FR, RTA1A, SLC4A1, SW, WD, WD1, WR
Calculated Molecular Weight 911 aa, 102 kDa
GenBank Accession NumberBC101570
Gene Symbol SLC4A1
Gene ID (NCBI) 6521
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02730
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 band 3/ AE1 antibody CL488-66984Download protocol
Standard Protocols
Click here to view our Standard Protocols