验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-80008-2 targets a-SMA specific in IF-P applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | actin, alpha 2, smooth muscle, aorta |
| 别名 | ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA |
| 计算分子量 | 42 kDa |
| GenBank蛋白编号 | NM_001613 |
| 基因名称 | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62736 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha smooth muscle actin (α-SMA or ACTA2) is an actin isoform predominantly expressed in smooth muscle cells and is the most widely recognized marker for activated myofibroblasts. In healthy adult tissues, its expression is largely restricted to vascular and visceral smooth muscle. However, during tissue injury, fibrosis, or wound healing, fibroblasts differentiate into myofibroblasts with significant upregulation of α-SMA. Therefore, α-SMA serves as a critical immunohistochemical marker for detecting myofibroblast activation and studying pathological processes such as organ fibrosis (e.g., liver, kidney, lung) and cancer stroma remodeling.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 a-SMA specific antibody CL647-80008-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
