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验证数据展示

  • WB analysis using 80008-2-RR (same clone as 80008-2-PBS)

    WB analysis using 80008-2-RR (same clone as 80008-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80008-2-RR (a-SMA specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 80008-2-RR (same clone as 80008-2-PBS)

    IHC staining of human colon using 80008-2-RR (same clone as 80008-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-2-RR (a-SMA specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human colon using 80008-2-RR (same clone as 80008-2-PBS)

    IHC staining of human colon using 80008-2-RR (same clone as 80008-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-2-RR (a-SMA specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IF Staining of mouse heart using 80008-2-RR (same clone as 80008-2-PBS)

    IF Staining of mouse heart using 80008-2-RR (same clone as 80008-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using a-SMA specific antibody (80008-2-RR, Clone: 250297G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 80008-2-RR (same clone as 80008-2-PBS)

    IF Staining of mouse heart using 80008-2-RR (same clone as 80008-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using a-SMA specific antibody (80008-2-RR, Clone: 250297G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 80008-2-RR (same clone as 80008-2-PBS)

    IF Staining of mouse kidney using 80008-2-RR (same clone as 80008-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using a-SMA specific antibody (80008-2-RR, Clone: 250297G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 80008-2-RR (same clone as 80008-2-PBS)

    IF Staining of mouse kidney using 80008-2-RR (same clone as 80008-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using a-SMA specific antibody (80008-2-RR, Clone: 250297G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-2-PBS in a different storage buffer formulation.

Alpha smooth muscle actin specific Recombinant monoclonal antibody, PBS Only

Alpha smooth muscle actin specific Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 80008-2-PBS

产品说明书

CloneNo. 250297G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.

产品信息

80008-2-PBS targets Alpha smooth muscle actin specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actin, alpha 2, smooth muscle, aorta
别名 ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA
计算分子量 42 kDa
观测分子量42 kDa
GenBank蛋白编号NM_001613
基因名称 Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_3743323
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62736
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha smooth muscle actin (α-SMA or ACTA2) is an actin isoform predominantly expressed in smooth muscle cells and is the most widely recognized marker for activated myofibroblasts. In healthy adult tissues, its expression is largely restricted to vascular and visceral smooth muscle. However, during tissue injury, fibrosis, or wound healing, fibroblasts differentiate into myofibroblasts with significant upregulation of α-SMA. Therefore, α-SMA serves as a critical immunohistochemical marker for detecting myofibroblast activation and studying pathological processes such as organ fibrosis (e.g., liver, kidney, lung) and cancer stroma remodeling.

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