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验证数据展示

  • IF Staining of PC-3 using CL488-84547-2

    IF Staining of PC-3 using CL488-84547-2

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite® Plus 488 ZMPSTE24 antibody (CL488-84547-2, Clone: 241288C3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ZMPSTE24 Recombinant monoclonal antibody

ZMPSTE24 Recombinant Antibody for IF/ICC
Cat No. CL488-84547-2

产品说明书

CloneNo. 241288C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy ZMPSTE24 Ready-To-Use IHC Kit. ZMPSTE24 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84547-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84547-2 targets ZMPSTE24 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag33613

Product name: Recombinant human ZMPSTE24 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 225-475 aa of BC037283

Sequence: TPLPEGKLKEEIEVMAKSIDFPLTKVYVVEGSKRSSHSNAYFYGFFKNKRIVLFDTLLEEYSVLNKDIQEDSGMEPRNEEEGNSEEIKAKVKNKKQGCKNEEVLAVLGHELGHWKLGHTVKNIIISQMNSFLCFFLFAVLIGRKELFAAFGFYDSQPTLIGLLIIFQFIFSPYNEVLSFCLTVLSRRFEFQADAFAKKLGKAKDLYSALIKLNKDNLGFPVSDWLFSMWHYSHPPLLERLQALKTMKQH*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc metallopeptidase (STE24 homolog, S. cerevisiae)
别名 FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog
计算分子量 475 aa, 55 kDa
GenBank蛋白编号BC037283
基因名称 ZMPSTE24
Gene ID (NCBI) 10269
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75844
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ZMPSTE24 antibody CL488-84547-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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