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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ZAP70 Monoclonal antibody

ZAP70 Monoclonal Antibody for FC (Intra), IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra), IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A11G9

Cat No : CL594-60200

Print datasheet

Synonyms

70 kDa zeta associated protein, FLJ17670, FLJ17679, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

验证数据展示

  • IF Staining of human tonsillitis using CL594-60200

    IF Staining of human tonsillitis using CL594-60200

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-60200 (ZAP70 antibody), at dilution of 1:100.

  • FC experiment of Jurkat using CL594-60200

    FC experiment of Jurkat using CL594-60200

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ZAP70 (CL594-60200, Clone:1A11G9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 60200-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-60200 is ZAP70 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF-P
Tested Reactivity human
Immunogen ZAP70 fusion protein Ag7943 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Synonyms 70 kDa zeta associated protein, FLJ17670, FLJ17679, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70
Calculated Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_2883439
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43403
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

实验方案

Product Specific Protocols
IF protocol for CL594 ZAP70 antibody CL594-60200Download protocol
FC protocol for CL594 ZAP70 antibody CL594-60200Download protocol
Standard Protocols
Click here to view our Standard Protocols