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ZAP70 Monoclonal antibody

ZAP70 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11G9

Cat No : 60200-1-Ig

Print datasheet

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

验证数据展示

  • WB analysis of Jurkat using 60200-1-Ig

    WB analysis of Jurkat using 60200-1-Ig

    WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells..

  • IF Staining of human tonsillitis using 60200-1-Ig

    IF Staining of human tonsillitis using 60200-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of Jurkat using 60200-1-Ig

    WB analysis of Jurkat using 60200-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 60200-1-Ig

    IHC staining of human lymphoma using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 60200-1-Ig

    IHC staining of human lymphoma using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, pig spleen tissue, pig thymus tissue
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 60200-1-Ig is ZAP70 Fusion Protein expressed in E. coli.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, pig
Cited Reactivitymouse
Immunogen ZAP70 fusion protein Ag7943 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Synonyms 70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_10944567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43403
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

实验方案

Product Specific Protocols
WB protocol for ZAP70 antibody 60200-1-IgDownload protocol
IHC protocol for ZAP70 antibody 60200-1-IgDownload protocol
IF protocol for ZAP70 antibody 60200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function

Authors - Yuman Wang
View Article