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验证数据展示

  • IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

    IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAB antibody included
  • IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

    IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAB antibody included
  • IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

    IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAB antibody included
  • IF Staining of HepG2 using 10936-1-AP (same clone as 10936-1-PBS)

    IF Staining of HepG2 using 10936-1-AP (same clone as 10936-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using YWHAB antibody (10936-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

YWHAB Polyclonal antibody, PBS Only

YWHAB Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 10936-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy YWHAB Ready-To-Use IHC Kit. YWHAB primary antibody included.

产品信息

10936-1-PBS targets YWHAB in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1383

Product name: Recombinant human YWHAB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-63 aa of BC010352

Sequence: MNDLICFLDNTFKNNVLSQAWWCVHLVPTIWEAEAGGSLEPRSLKLQCPVVAPVNNCTPAWAT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
别名
计算分子量 28 kDa
GenBank蛋白编号BC010352
基因名称 YWHAB
Gene ID (NCBI) 7529
RRIDAB_2217489
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31946
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YWHAB (also known as 14-3-3 beta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised agaist the N-terminal region of human YWHAB.

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