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验证数据展示

  • IF Staining of PC-12 using CL488-25537

    IF Staining of PC-12 using CL488-25537

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 YTHDF3-specific antibody (CL488-25537) at dilution of 1:200.

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  • KD/KO验证

CoraLite® Plus 488-conjugated YTHDF3-specific Polyclonal antibody

YTHDF3-specific Polyclonal Antibody for IF/ICC
Cat No. CL488-25537

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

FLJ31657, YTH domain family protein 3, YTH domain family, member 3, YTHDF3

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF3 Ready-To-Use IHC Kit. YTHDF3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25537-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25537 targets YTHDF3-specific in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22374

Product name: Recombinant human YTHDF3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 284-351 aa of BC052970

Sequence: SVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YTH domain family, member 3
别名 FLJ31657, YTH domain family protein 3, YTH domain family, member 3, YTHDF3
计算分子量 585 aa, 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC052970
基因名称 YTHDF3
Gene ID (NCBI) 253943
RRIDAB_3672794
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z739
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 YTHDF3-specific antibody CL488-25537Download protocol
Standard Protocols
Click here to view our Standard Protocols
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