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YTHDF3-specific Polyclonal antibody
YTHDF3-specific Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
IF/ICC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HCT 116 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | PC-12 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25537-1-AP targets YTHDF3-specific in WB, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, RIP, IHC, CoIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, chicken, pig |
Immunogen | YTHDF3-specific fusion protein Ag22374 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | YTH domain family, member 3 |
Synonyms | YTH domain family protein 3, YTH domain family, member 3, YTHDF3, YTHDF3-specific |
Calculated Molecular Weight | 585 aa, 64 kDa |
Observed Molecular Weight | 64 kDa |
GenBank Accession Number | BC052970 |
Gene Symbol | YTHDF3 |
Gene ID (NCBI) | 253943 |
RRID | AB_2847817 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q7Z739 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
IHC protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
IF protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis
| ||
Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. | ||
Nat Commun Circular RNA encoded MET variant promotes glioblastoma tumorigenesis
| ||
Adv Sci (Weinh) RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation
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