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验证数据展示

  • WB analysis of Jurkat using 24744-1-AP

    WB analysis of Jurkat using 24744-1-AP

    WB result of YTHDF2 antibody (24744-1-AP, 1:4000) with si-control and si-YTHDF2 transfected Jurkat cells..

  • WB analysis using 24744-1-AP

    WB analysis using 24744-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 24744-1-AP

    IF Staining of U2OS using 24744-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of HeLa using 24744-1-AP

    IP experiment of HeLa using 24744-1-AP

    IP result of anti-YTHDF2 (IP:24744-1-AP, 4ug; Detection:24744-1-AP 1:6000) with HeLa cells lysate 1360 ug.

  • IF Staining of mouse brain using 24744-1-AP

    IF Staining of mouse brain using 24744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24744-1-AP

    IF Staining of HeLa using 24744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

  • IF Staining of U2OS using 24744-1-AP

    IF Staining of U2OS using 24744-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA
Cat No. 24744-1-AP

产品说明书

引用文献(327)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (6)

应用

WB, IF/ICC, IF-P, IP, ELISA and More (6)

别名

YTH domain family protein 2, NY REN 2, HGRG8, DF2, CLL-associated antigen KW-14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, MOLT-4 cells, NIH/3T3 cells, C6 cells, Raji cells
Positive IP detected inHeLa cells
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inU2OS cells, HeLa cells, sodium arsenite treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF2 Ready-To-Use IHC Kit. YTHDF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24744-1-AP targets YTHDF2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, chIP, RIP, ELISA, CLIP applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, chIP, RIP, CLIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey, chicken, bovine, sheep, goat
免疫原 YTHDF2 fusion protein Ag5922 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YTH domain family, member 2
别名 YTH domain family protein 2, NY REN 2, HGRG8, DF2, CLL-associated antigen KW-14
计算分子量 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC002559
基因名称 YTHDF2
Gene ID (NCBI) 51441
RRIDAB_2687435
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5A9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

实验方案

Product Specific Protocols
WB protocol for YTHDF2 antibody 24744-1-APDownload protocol
IF protocol for YTHDF2 antibody 24744-1-APDownload protocol
IP protocol for YTHDF2 antibody 24744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

m6A enhances the phase separation potential of mRNA.

Authors - Ryan J Ries
View Article
mouseRIP

Nature

m6A RNA methylation regulates the fate of endogenous retroviruses.

Authors - Tomasz Chelmicki
  • KO Validated
View Article
mouseWB

Nature

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi
View Article
human, mouseWB, IF

Nature

Dynamic m(6)A mRNA methylation directs translational control of heat shock response.

Authors - Jun Zhou
View Article
humanWB

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
View Article
human,mouseFC

Nat Med

Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.

Authors - Irene Ganan-Gomez
View Article