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验证数据展示

  • WB analysis of Jurkat using 24744-1-AP (same clone as 24744-1-PBS)

    WB analysis of Jurkat using 24744-1-AP (same clone as 24744-1-PBS)

    WB result of YTHDF2 antibody (24744-1-AP, 1:4000) with si-control and si-YTHDF2 transfected Jurkat cells.. This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

  • WB analysis using 24744-1-AP (same clone as 24744-1-PBS)

    WB analysis using 24744-1-AP (same clone as 24744-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 24744-1-AP (same clone as 24744-1-PBS)

    IF Staining of U2OS using 24744-1-AP (same clone as 24744-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 24744-1-AP (same clone as 24744-1-PBS)

    IP experiment of HeLa using 24744-1-AP (same clone as 24744-1-PBS)

    IP result of anti-YTHDF2 (IP:24744-1-AP, 4ug; Detection:24744-1-AP 1:6000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 24744-1-AP (same clone as 24744-1-PBS)

    IHC staining of mouse brain using 24744-1-AP (same clone as 24744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24744-1-AP (YTHDF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF2 antibody included
  • IHC staining of mouse brain using 24744-1-AP (same clone as 24744-1-PBS)

    IHC staining of mouse brain using 24744-1-AP (same clone as 24744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24744-1-AP (YTHDF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF2 antibody included
  • IF Staining of mouse brain using 24744-1-AP (same clone as 24744-1-PBS)

    IF Staining of mouse brain using 24744-1-AP (same clone as 24744-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 24744-1-AP (same clone as 24744-1-PBS)

    IF Staining of HeLa using 24744-1-AP (same clone as 24744-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 24744-1-AP (same clone as 24744-1-PBS)

    IF Staining of U2OS using 24744-1-AP (same clone as 24744-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24744-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

YTHDF2 Polyclonal antibody, PBS Only

YTHDF2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 24744-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA

别名

CLL associated antigen KW 14, CLL-associated antigen KW-14, DF2, HGRG8, NY REN 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy YTHDF2 Ready-To-Use IHC Kit. YTHDF2 primary antibody included.

产品信息

24744-1-PBS targets YTHDF2 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5922

Product name: Recombinant human YTHDF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 339-565 aa of BC002559

Sequence: LSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YTH domain family, member 2
别名 CLL associated antigen KW 14, CLL-associated antigen KW-14, DF2, HGRG8, NY REN 2
计算分子量 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC002559
基因名称 YTHDF2
Gene ID (NCBI) 51441
RRIDAB_2687435
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5A9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity. Due to the high sequence similarity, 24744-1-AP is likely to cross-react with both YTHDF1 and YTHDF3.

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