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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated YME1L1 Polyclonal antibody

YME1L1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-11510

Print datasheet

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1

验证数据展示

  • IF Staining of MCF-7 using CL594-11510

    IF Staining of MCF-7 using CL594-11510

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 YME1L1 antibody (CL594-11510) at dilution of 1:200.

经过测试的应用

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 11510-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-11510 targets YME1L1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen YME1L1 fusion protein Ag2069 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name YME1-like 1 (S. cerevisiae)
Synonyms FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1
Calculated Molecular Weight 716 aa, 80 kDa
Observed Molecular Weight 86 kDa, 63 kDa
GenBank Accession NumberBC023507
Gene Symbol YME1L1
Gene ID (NCBI) 10730
RRIDAB_2919765
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96TA2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

实验方案

Product Specific Protocols
IF protocol for CL594 YME1L1 antibody CL594-11510Download protocol
Standard Protocols
Click here to view our Standard Protocols