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  • KD/KO Validated

YME1L1 Polyclonal antibody

YME1L1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11510-1-AP

Print datasheet

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1



经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11510-1-AP targets YME1L1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Immunogen YME1L1 fusion protein Ag2069 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name YME1-like 1 (S. cerevisiae)
Synonyms FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1
Calculated Molecular Weight 716 aa, 80 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC023507
Gene Symbol YME1L1
Gene ID (NCBI) 10730
RRIDAB_2217459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96TA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

实验方案

Product Specific Protocols
WB protocol for YME1L1 antibody 11510-1-APDownload protocol
IF protocol for YME1L1 antibody 11510-1-APDownload protocol
IP protocol for YME1L1 antibody 11510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Authors - Tao Wang
  • KD Validated
humanWB

Cell Metab

A High-Density Human Mitochondrial Proximity Interaction Network.

Authors - Hana Antonicka
monkeyWB

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
  • KD Validated
humanWB

Nat Cell Biol

PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.

Authors - Shotaro Saita
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
humanIF

Mol Cell

Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress.

Authors - Raul Jobava