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CoraLite® Plus 488-conjugated XPA Polyclonal antibody

XPA Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-16462

Print datasheet

Synonyms

XP1, XPA, XPAC

验证数据展示

  • FC experiment of HepG2 using CL488-16462

    FC experiment of HepG2 using CL488-16462

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human XPA (CL488-16462) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16462-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16462 targets XPA in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse
Immunogen XPA fusion protein Ag9536 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name xeroderma pigmentosum, complementation group A
Synonyms XP1, XPA, XPAC
Calculated Molecular Weight374aa,42 kDa; 273aa,31 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC014965
Gene Symbol XPA
Gene ID (NCBI) 7507
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23025
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 XPA antibody CL488-16462Download protocol
Standard Protocols
Click here to view our Standard Protocols