验证数据展示
经过测试的应用
| Positive IHC detected in | 293T cell（218Linker） Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Transfected CHO cells |
| Positive FC detected in | Transfected HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98672-3-RR targets Whitlow/218 Linker in IHC, IF/ICC, FC applications and shows reactivity with n/a samples.
| 经测试应用 | IHC, IF/ICC, FC Application Description |
| 经测试反应性 | n/a |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Whitlow/218 Linker |
| 别名 | 218, Linker, Whitlow, Whitlow Linker, 218 Linker |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
The 218 Linker (also known as the Whitlow linker) is a synthetic peptide sequence, connecting two protein domains, most commonly the Variable Heavy (VH) and Variable Light (VL) chains of an antibody, to form a stable single-chain variable fragment (scFv) (PMID: 8309948). Its standard amino acid sequence is GSTSGSGKPGSGEGSTKG.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Whitlow/218 Linker antibody 98672-3-RR | Download protocol |
| IF protocol for Whitlow/218 Linker antibody 98672-3-RR | Download protocol |
| IHC protocol for Whitlow/218 Linker antibody 98672-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
