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验证数据展示

  • FC experiment of Transfected HEK-293T using 98672-3-RR (same clone as 98672-3-PBS)

    FC experiment of Transfected HEK-293T using 98672-3-RR (same clone as 98672-3-PBS)

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8a hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti-Whitlow/218 Linker Rabbit RecAb (98672-3-RR, Clone:252007G8), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9),and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98672-3-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 98672-3-RR (same clone as 98672-3-PBS)

    FC experiment of Transfected HEK-293T using 98672-3-RR (same clone as 98672-3-PBS)

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8a hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti-Whitlow/218 Linker Rabbit RecAb (98672-3-RR, Clone:252007G8) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98672-3-PBS in a different storage buffer formulation.

  • IHC staining of 293T cell（218Linker） using 98672-3-RR (same clone as 98672-3-PBS)

    IHC staining of 293T cell（218Linker） using 98672-3-RR (same clone as 98672-3-PBS)

    Immunohistochemical analysis of paraffin-embedded transfected HEK-293T cells slide using 98672-3-RR (Whitlow/218 Linker antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98672-3-PBS in a different storage buffer formulation.

  • IF Staining of Transfected CHO using 98672-3-RR (same clone as 98672-3-PBS)

    IF Staining of Transfected CHO using 98672-3-RR (same clone as 98672-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected CHO cells using 218linker antibody (98672-3-RR, Clone: 252007G1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 98672-3-PBS in a different storage buffer formulation.

Anti-Whitlow/218 Linker Rabbit Recombinant Antibody, PBS Only

Whitlow/218 Linker Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC
Cat No. 98672-3-PBS

产品说明书

CloneNo. 252007G1

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

IHC, IF/ICC, FC

别名

218, Linker, Whitlow, Whitlow Linker, 218 Linker

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98672-3-PBS targets Whitlow/218 Linker in IHC, IF/ICC, FC applications and shows reactivity with n/a samples.

经测试应用 IHC, IF/ICC, FC Application Description
经测试反应性 n/a
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Whitlow/218 Linker
别名 218, Linker, Whitlow, Whitlow Linker, 218 Linker
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 218 Linker (also known as the Whitlow linker) is a synthetic peptide sequence, connecting two protein domains, most commonly the Variable Heavy (VH) and Variable Light (VL) chains of an antibody, to form a stable single-chain variable fragment (scFv) (PMID: 8309948). Its standard amino acid sequence is GSTSGSGKPGSGEGSTKG.

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