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验证数据展示

  • WB analysis using 67725-1-Ig (same clone as 67725-1-PBS)

    WB analysis using 67725-1-Ig (same clone as 67725-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67725-1-Ig (WWOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67725-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67725-1-Ig (same clone as 67725-1-PBS)

    IHC staining of mouse testis using 67725-1-Ig (same clone as 67725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67725-1-Ig (WWOX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67725-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WWOX antibody included
  • Cytometric bead array standard curve of MP51258-1

    Cytometric bead array standard curve of MP51258-1

    Cytometric bead array standard curve of MP51258-1, WWOX Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67725-2-PBS. Detection antibody: 67725-1-PBS. Standard:Ag7969. Range: 0.195-100 ng/mL.

WWOX Monoclonal antibody, PBS Only (Detector)

WWOX Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67725-1-PBS

产品说明书

CloneNo. 1B8F4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

FRA16D, FOR1, FOR, EC:1.1.1.-, D16S432E

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy WWOX Ready-To-Use IHC Kit. WWOX primary antibody included.

产品信息

67725-1-PBS targets WWOX as part of a matched antibody pair:

MP51258-1: 67725-2-PBS capture and 67725-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 WWOX fusion protein Ag7969 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 WW domain containing oxidoreductase
别名 FRA16D, FOR1, FOR, EC:1.1.1.-, D16S432E
计算分子量 47 kDa
观测分子量40 kDa
GenBank蛋白编号BC003184
基因名称 WWOX
Gene ID (NCBI) 51741
RRIDAB_2882911
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZC7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.