• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67725-1-Ig

    WB analysis using 67725-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67725-1-Ig (WWOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 67725-1-Ig

    IHC staining of mouse testis using 67725-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67725-1-Ig (WWOX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WWOX antibody included

WWOX Monoclonal antibody

WWOX Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67725-1-Ig

产品说明书

CloneNo. 1B8F4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, pig, mouse

应用

WB, IHC, ELISA

别名

D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, pig liver tissue, rat liver tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy WWOX Ready-To-Use IHC Kit. WWOX primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67725-1-Ig targets WWOX in WB, IHC, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat, pig, mouse
免疫原 WWOX fusion protein Ag7969 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 WW domain containing oxidoreductase
别名 D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX
计算分子量 47 kDa
观测分子量40 kDa
GenBank蛋白编号BC003184
基因名称 WWOX
Gene ID (NCBI) 51741
RRIDAB_2882911
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for WWOX antibody 67725-1-IgDownload protocol
IHC protocol for WWOX antibody 67725-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols