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验证数据展示

  • FC experiment of HeLa using 84577-4-RR

    FC experiment of HeLa using 84577-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug WNT3A Recombinant antibody (84577-4-RR, Clone:241993E8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 84577-4-RR

    IF Staining of HepG2 using 84577-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WNT3A antibody (84577-4-RR, Clone: 241993E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84577-4-RR

    Affinity and Kinetic Characterization of 84577-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84577-4-RR against Human WNT3A were performed. The affinity constant is 4.67 nM.

WNT3A Recombinant antibody

WNT3A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84577-4-RR

产品说明书

CloneNo. 241993E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Protein Wnt-3a, Protein Wnt 3a, 241993E8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84577-4-RR targets WNT3A in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 WNT3A fusion protein Ag25051 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 3A
别名 Protein Wnt-3a, Protein Wnt 3a, 241993E8
计算分子量 352 aa, 39 kDa
GenBank蛋白编号BC103921
基因名称 WNT3A
Gene ID (NCBI) 89780
RRIDAB_3672077
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP56704
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders (PMID: 20716463). Wnt3a, was shown to promote trophectoderm formation in embryonic stem cells by inducing Cdx2, one of the critical transcription factors of early trophoblast development, in an LEF-1-dependent manner. Wnt3a may also activate other β-catenin-independent cascades such as ERK or PI3K/AKT signalling, again emphasizing the complexity of the Wnt signalling network.

实验方案

Product Specific Protocols
IF protocol for WNT3A antibody 84577-4-RRDownload protocol
FC protocol for WNT3A antibody 84577-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols