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验证数据展示

  • FC experiment of HeLa using 84577-4-RR (same clone as 84577-4-PBS)

    FC experiment of HeLa using 84577-4-RR (same clone as 84577-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug WNT3A Recombinant antibody (84577-4-RR, Clone:241993E8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84577-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84577-4-RR (same clone as 84577-4-PBS)

    IF Staining of HepG2 using 84577-4-RR (same clone as 84577-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WNT3A antibody (84577-4-RR, Clone: 241993E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84577-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84577-4-RR

    Affinity and Kinetic Characterization of 84577-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84577-4-RR against Human WNT3A were performed. The affinity constant is 4.67 nM.

WNT3A Recombinant antibody, PBS Only

WNT3A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84577-4-PBS

产品说明书

CloneNo. 241993E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

Protein Wnt-3a, Protein Wnt 3a, 241993E8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84577-4-PBS targets WNT3A in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 WNT3A fusion protein Ag25051 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 3A
别名 Protein Wnt-3a, Protein Wnt 3a, 241993E8
计算分子量 352 aa, 39 kDa
GenBank蛋白编号BC103921
基因名称 WNT3A
Gene ID (NCBI) 89780
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP56704
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders (PMID: 20716463). Wnt3a, was shown to promote trophectoderm formation in embryonic stem cells by inducing Cdx2, one of the critical transcription factors of early trophoblast development, in an LEF-1-dependent manner. Wnt3a may also activate other β-catenin-independent cascades such as ERK or PI3K/AKT signalling, again emphasizing the complexity of the Wnt signalling network.