CoraLite® Plus 488-conjugated WNT10B Monoclonal antibody
WNT10B Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G11E4
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy WNT10B Ready-To-Use IHC Kit. WNT10B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67210-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67210 targets WNT10B in IF, FC (Intra) applications and shows reactivity with Human samples.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | Human |
Immunogen | WNT10B fusion protein Ag17114 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | wingless-type MMTV integration site family, member 10B |
Synonyms | Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10B, WNT12 |
Calculated Molecular Weight | 389 aa, 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC096353 |
Gene Symbol | WNT10B |
Gene ID (NCBI) | 7480 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O00744 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 WNT10B antibody CL488-67210 | Download protocol |
FC protocol for CL Plus 488 WNT10B antibody CL488-67210 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |