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验证数据展示

  • IHC staining of mouse testis using 23404-1-AP (same clone as 23404-1-PBS)

    IHC staining of mouse testis using 23404-1-AP (same clone as 23404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23404-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 23404-1-AP (same clone as 23404-1-PBS)

    IF Staining of hTERT-RPE1 using 23404-1-AP (same clone as 23404-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23404-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 23404-1-AP (same clone as 23404-1-PBS)

    IHC staining of mouse testis using 23404-1-AP (same clone as 23404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23404-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 23404-1-AP (same clone as 23404-1-PBS)

    IF Staining of A549 using 23404-1-AP (same clone as 23404-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23404-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293T using 23404-1-AP (same clone as 23404-1-PBS)

    IP experiment of HEK-293T using 23404-1-AP (same clone as 23404-1-PBS)

    IP result of anti-WDR62 (IP:23404-1-AP, 4ug; Detection:23404-1-AP 1:500) with HEK-293T cells lysate 1320 ug. This data was developed using the same antibody clone with 23404-1-PBS in a different storage buffer formulation.

WDR62 Polyclonal antibody, PBS Only

WDR62 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 23404-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IP, Indirect ELISA

别名

C19orf14, WD repeat domain 62, WD repeat-containing protein 62

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

23404-1-PBS targets WDR62 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19865

Product name: Recombinant human WDR62 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-630 aa of BC017261

Sequence: CLTSLASCVPASSVLPTDRNLPTPTSAPTPGLAQGVHAPSTCSYMEATASSRARISRSISLGDSEGPIVATLAQPLRRPSSVGELASLGQELQAITTATTPSLDSEGQEPALRSWGNHEARANLRLTLSSACDGLLLPPVDTQPGVTVPAVSFPAPSPVEESALRLHGSAFRPSLPAPESPGLPAHPSNPQLPEARPGIPGSTASLLEPTSGALGLFQGSPARWSEPWVPVEALPPSPLELSRVGNILHRLQTTFQEALDLYRVLVSSGQVDTGQQQARTELVSTFLWIHSQLEAECLVGTSVAPAQALPSPGPPSPPTLYPLASPDLQALLEHYSELLVQAVRRKARGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 62
别名 C19orf14, WD repeat domain 62, WD repeat-containing protein 62
计算分子量 1518 aa, 166 kDa
观测分子量180 kDa
GenBank蛋白编号BC017261
基因名称 WDR62
Gene ID (NCBI) 284403
RRIDAB_3085711
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43379
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The WD40-repeat protein 62 (WDR62) is a scaffold protein that recruits different components of the JNK signaling pathway to regulate several human diseases including neurological disorders, infertility, and tumorigenesis (PMID: 33937237). Moreover, WDR62 participates in diverse biological processes, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein (PMID: 35093571).

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