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WDR62 Polyclonal antibody

WDR62 Polyclonal Antibody for IF/ICC, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, ELISA

Conjugate

Unconjugated

Cat No : 23404-1-AP

Print datasheet

Synonyms

C19orf14, WD repeat domain 62, WDR62

验证数据展示

  • IP experiment of HEK-293T using 23404-1-AP

    IP experiment of HEK-293T using 23404-1-AP

    IP result of anti-WDR62 (IP:23404-1-AP, 4ug; Detection:23404-1-AP 1:300) with HEK-293T cells lysate 1560 ug.

  • IHC staining of mouse testis using 23404-1-AP

    IHC staining of mouse testis using 23404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of hTERT-RPE1 using 23404-1-AP

    IF Staining of hTERT-RPE1 using 23404-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 23404-1-AP

    IHC staining of mouse testis using 23404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 23404-1-AP

    IF Staining of A549 using 23404-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IP detected inHEK-293T cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, A549 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23404-1-AP targets WDR62 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, ELISA
Tested Reactivity human, mouse
Immunogen WDR62 fusion protein Ag19865 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 62
Synonyms C19orf14, WD repeat domain 62, WDR62
Calculated Molecular Weight 1518 aa, 166 kDa
GenBank Accession NumberBC017261
Gene Symbol WDR62
Gene ID (NCBI) 284403
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO43379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The WD40-repeat protein 62 (WDR62) is a scaffold protein that recruits different components of the JNK signaling pathway to regulate several human diseases including neurological disorders, infertility, and tumorigenesis (PMID: 33937237). Moreover, WDR62 participates in diverse biological processes, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein (PMID: 35093571).

实验方案

Product Specific Protocols
IHC protocol for WDR62 antibody 23404-1-APDownload protocol
IF protocol for WDR62 antibody 23404-1-APDownload protocol
IP protocol for WDR62 antibody 23404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols