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验证数据展示

  • WB analysis using 30227-1-AP (same clone as 30227-1-PBS)

    WB analysis using 30227-1-AP (same clone as 30227-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30227-1-AP (WDR43 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 30227-1-AP (same clone as 30227-1-PBS)

    IHC staining of mouse lung using 30227-1-AP (same clone as 30227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 30227-1-AP (same clone as 30227-1-PBS)

    IHC staining of mouse ovary using 30227-1-AP (same clone as 30227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 30227-1-AP (same clone as 30227-1-PBS)

    IHC staining of rat liver using 30227-1-AP (same clone as 30227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • IHC staining of rat ovary using 30227-1-AP (same clone as 30227-1-PBS)

    IHC staining of rat ovary using 30227-1-AP (same clone as 30227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 30227-1-AP (same clone as 30227-1-PBS)

    IF Staining of HeLa using 30227-1-AP (same clone as 30227-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR43 antibody (30227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

WDR43 Polyclonal antibody, PBS Only

WDR43 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30227-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA0007, WD repeat domain 43

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30227-1-PBS targets WDR43 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30585

Product name: Recombinant human WDR43 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-150 aa of NM_015131

Sequence: MAAGGGGSCDPLAPAGVPCAFSPHSQAYFALASTDGHLRVWETANNRLHQEYVPSAHLSGTCTCLAWAPARLQAKESPQRKKRKSEAVGMSNQTDLLALGTAVGSILLYSTVKGELHSKLISGGHDNRVNCIQWHQDSGCLYSCSDDKHI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 43
别名 KIAA0007, WD repeat domain 43
计算分子量 75 kDa
观测分子量75 kDa
GenBank蛋白编号NM_015131
基因名称 WDR43
Gene ID (NCBI) 23160
RRIDAB_3086268
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15061
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WDR43, a WD40 domain-containing protein, is a conserved component of the small-subunit processome (SSUP), which mediates pre-18S ribosomal RNA (rRNA) transcription and processing in the nucleolus and is critical for ribosome biogenesis in yeast. Its mutation in zebrafish causes a variety of early developmental defects in specific tissues. WDR43 directly promotes ESC self-renewal by maintaining high-level expression of its target genes involved in pluripotency programs. (PMID: 28880863, 24497835, 31128943)

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