验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30227-1-AP targets WDR43 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | WDR43 fusion protein Ag30585 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 43 |
| 别名 | KIAA0007, WD repeat domain 43, WDR43 |
| 计算分子量 | 75 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | NM_015131 |
| 基因名称 | WDR43 |
| Gene ID (NCBI) | 23160 |
| RRID | AB_3086268 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15061 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR43, a WD40 domain-containing protein, is a conserved component of the small-subunit processome (SSUP), which mediates pre-18S ribosomal RNA (rRNA) transcription and processing in the nucleolus and is critical for ribosome biogenesis in yeast. Its mutation in zebrafish causes a variety of early developmental defects in specific tissues. WDR43 directly promotes ESC self-renewal by maintaining high-level expression of its target genes involved in pluripotency programs. (PMID: 28880863, 24497835, 31128943)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR43 antibody 30227-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |