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验证数据展示

  • WB analysis using 30227-1-AP

    WB analysis using 30227-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30227-1-AP (WDR43 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 30227-1-AP

    IHC staining of mouse lung using 30227-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse ovary using 30227-1-AP

    IHC staining of mouse ovary using 30227-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 30227-1-AP

    IHC staining of rat liver using 30227-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat ovary using 30227-1-AP

    IHC staining of rat ovary using 30227-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 30227-1-AP

    IF Staining of HeLa using 30227-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR43 antibody (30227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

WDR43 Polyclonal antibody

WDR43 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30227-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA0007, WD repeat domain 43

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse lung tissue, mouse ovary tissue, rat liver tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

30227-1-AP targets WDR43 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag30585

Product name: Recombinant human WDR43 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-150 aa of NM_015131

Sequence: MAAGGGGSCDPLAPAGVPCAFSPHSQAYFALASTDGHLRVWETANNRLHQEYVPSAHLSGTCTCLAWAPARLQAKESPQRKKRKSEAVGMSNQTDLLALGTAVGSILLYSTVKGELHSKLISGGHDNRVNCIQWHQDSGCLYSCSDDKHI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 43
别名 KIAA0007, WD repeat domain 43
计算分子量 75 kDa
观测分子量75 kDa
GenBank蛋白编号NM_015131
基因名称 WDR43
Gene ID (NCBI) 23160
RRIDAB_3086268
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15061
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR43, a WD40 domain-containing protein, is a conserved component of the small-subunit processome (SSUP), which mediates pre-18S ribosomal RNA (rRNA) transcription and processing in the nucleolus and is critical for ribosome biogenesis in yeast. Its mutation in zebrafish causes a variety of early developmental defects in specific tissues. WDR43 directly promotes ESC self-renewal by maintaining high-level expression of its target genes involved in pluripotency programs. (PMID: 28880863, 24497835, 31128943)

实验方案

Product Specific Protocols
IF protocol for WDR43 antibody 30227-1-APDownload protocol
IHC protocol for WDR43 antibody 30227-1-APDownload protocol
WB protocol for WDR43 antibody 30227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cancer Discov

ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translation

Authors - Chao Shen
View Article
humanWB

Sci Adv

Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.

Authors - Paulina Podszywałow-Bartnicka
View Article
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