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验证数据展示

  • WB analysis using 86464-1-RR

    WB analysis using 86464-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86464-1-RR (WDR24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 86464-1-RR

    WB analysis of HEK-293 using 86464-1-RR

    WB result of WDR24 antibody (86464-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells.

  • IP experiment of HEK-293 using 86464-1-RR

    IP experiment of HEK-293 using 86464-1-RR

    IP result of anti-WDR24 (IP:86464-1-RR, 4ug; Detection:86464-1-RR 1:2000) with HEK-293 cells lysate 1200 ug.

  • Affinity and Kinetic Characterization of 86464-1-RR

    Affinity and Kinetic Characterization of 86464-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86464-1-RR against Human WDR24 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

WDR24 Recombinant monoclonal antibody

WDR24 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86464-1-RR

产品说明书

CloneNo. 251220G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C16orf21, EC:2.3.2.27, GATOR2 complex protein WDR24, JFP7, WD repeat-containing protein 24

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, HuH-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86464-1-RR targets WDR24 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14774

Product name: Recombinant human WDR24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 440-790 aa of BC008025

Sequence: LGRNQVAQTWTMLRIIYCSPGLVPTANLNHSVGKGGSCGLPLMNSFNLKDMAPGLGSETRLDRSKGDARSDTVLLDSSATLITNEDNEETEGSDVPADYLLGDVEGEEDELYLLDPEHAHPEDPECVLPQEAFPLRHEIVDTPPGPEHLQDKADSPHVSGSEADVASLAPVDSSFSLLSVSHALYDSRLPPDFFGVLVRDMLHFYAEQGDVQMAVSVLIVLGERVRKDIDEQTQEHWYTSYIDLLQRFRLWNVSNEVVKLSTSRAVSCLNQASTTLHVNCSHCKRPMSSRGWVCDRCHRCASMCAVCHHVVKGLFVWCQGCSHGGHLQHIMKWLEGSSHCPAGCGHLCEYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 WD repeat domain 24
别名 C16orf21, EC:2.3.2.27, GATOR2 complex protein WDR24, JFP7, WD repeat-containing protein 24
计算分子量 920 aa, 102 kDa
观测分子量88 kDa
GenBank蛋白编号BC008025
基因名称 WDR24
Gene ID (NCBI) 84219
RRIDAB_3744904
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96S15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

实验方案

Product Specific Protocols
IP protocol for WDR24 antibody 86464-1-RRDownload protocol
WB protocol for WDR24 antibody 86464-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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