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验证数据展示

  • WB analysis using 86464-1-RR (same clone as 86464-1-PBS)

    WB analysis using 86464-1-RR (same clone as 86464-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86464-1-RR (WDR24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86464-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 86464-1-RR (same clone as 86464-1-PBS)

    WB analysis of HEK-293 using 86464-1-RR (same clone as 86464-1-PBS)

    WB result of WDR24 antibody (86464-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells. This data was developed using the same antibody clone with 86464-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 86464-1-RR (same clone as 86464-1-PBS)

    IP experiment of HEK-293 using 86464-1-RR (same clone as 86464-1-PBS)

    IP result of anti-WDR24 (IP:86464-1-RR, 4ug; Detection:86464-1-RR 1:2000) with HEK-293 cells lysate 1200 ug. This data was developed using the same antibody clone with 86464-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86464-1-RR

    Affinity and Kinetic Characterization of 86464-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86464-1-RR against Human WDR24 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

WDR24 Recombinant monoclonal antibody, PBS Only

WDR24 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86464-1-PBS

产品说明书

CloneNo. 251220G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

C16orf21, EC:2.3.2.27, GATOR2 complex protein WDR24, JFP7, WD repeat-containing protein 24

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86464-1-PBS targets WDR24 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14774

Product name: Recombinant human WDR24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 440-790 aa of BC008025

Sequence: LGRNQVAQTWTMLRIIYCSPGLVPTANLNHSVGKGGSCGLPLMNSFNLKDMAPGLGSETRLDRSKGDARSDTVLLDSSATLITNEDNEETEGSDVPADYLLGDVEGEEDELYLLDPEHAHPEDPECVLPQEAFPLRHEIVDTPPGPEHLQDKADSPHVSGSEADVASLAPVDSSFSLLSVSHALYDSRLPPDFFGVLVRDMLHFYAEQGDVQMAVSVLIVLGERVRKDIDEQTQEHWYTSYIDLLQRFRLWNVSNEVVKLSTSRAVSCLNQASTTLHVNCSHCKRPMSSRGWVCDRCHRCASMCAVCHHVVKGLFVWCQGCSHGGHLQHIMKWLEGSSHCPAGCGHLCEYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 WD repeat domain 24
别名 C16orf21, EC:2.3.2.27, GATOR2 complex protein WDR24, JFP7, WD repeat-containing protein 24
计算分子量 920 aa, 102 kDa
观测分子量88 kDa
GenBank蛋白编号BC008025
基因名称 WDR24
Gene ID (NCBI) 84219
RRIDAB_3744904
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96S15
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

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