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验证数据展示

  • WB analysis using 31442-1-AP (same clone as 31442-1-PBS)

    WB analysis using 31442-1-AP (same clone as 31442-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31442-1-AP (WASF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31442-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 31442-1-AP (same clone as 31442-1-PBS)

    IHC staining of human placenta using 31442-1-AP (same clone as 31442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31442-1-AP (WASF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31442-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 31442-1-AP (same clone as 31442-1-PBS)

    IF Staining of U2OS using 31442-1-AP (same clone as 31442-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using WASF2 antibody (31442-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31442-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 31442-1-AP (same clone as 31442-1-PBS)

    WB analysis of U2OS using 31442-1-AP (same clone as 31442-1-PBS)

    WB result of WASF2 antibody (31442-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WASF2 transfected U2OS cells. This data was developed using the same antibody clone with 31442-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WASF2 Polyclonal antibody, PBS Only

WASF2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31442-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Protein WAVE 2, SCAR2, WAS protein family, member 2, WASP family protein member 2, WAVE2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31442-1-PBS targets WASF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35663

Product name: Recombinant human WASF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 182-315 aa of NM_006990.4

Sequence: KKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WAS protein family, member 2
别名 Protein WAVE 2, SCAR2, WAS protein family, member 2, WASP family protein member 2, WAVE2
计算分子量54kDa，498aa
观测分子量80 kDa
GenBank蛋白编号NM_006990.4
基因名称 WASF2
Gene ID (NCBI) 10163
RRIDAB_3669985
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y6W5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WASF2 (WASp family Verprolin-homologous protein-2), also known as WAVE2, a member of the Wiskott-Aldrich syndrome protein (WASp) family of actin nucleation promoting factors, is a downstream effector molecule that implicates the signal transduction from small GTPases to the actin cytoskeleton, which can elongate the cell movement mode (PMID: 35849030,28332566). Moreover, WASF2 promoted the invasiveness of cancer cells by binding to actin cytoskeletal protein alpha-actinin 4 (ACTN4) (PMID: 30353690). The calculated MW of WASF2 is 54 kDa, with an isoform of 32 kDa, but the observed MW is about 80 kDa, which may be due to post-translational modification (PMID: 35849030,28332566,21383498,17389688,18362183,38391912).

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